In traditional 2D monolayer cultures, exogenous soluble factors or cell-secreted endogenous factors diffuse freely throughout the medium, and thereby reach an equilibrium in which all cells are exposed to similar biochemical environments.5 In contrast, in 3D aggregate Tenofovir (Viread) cultures, a concentration gradient is established between the surrounding culture environment and the interior of the … Continue reading In traditional 2D monolayer cultures, exogenous soluble factors or cell-secreted endogenous factors diffuse freely throughout the medium, and thereby reach an equilibrium in which all cells are exposed to similar biochemical environments
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